High nucleotide sequence variation of avirulent gene, AVR-Pita1, in Thai rice blast fungus population.
Identifieur interne : 000142 ( Main/Exploration ); précédent : 000141; suivant : 000143High nucleotide sequence variation of avirulent gene, AVR-Pita1, in Thai rice blast fungus population.
Auteurs : Katanyutita Damchuay [Thaïlande] ; Apinya Longya ; Tanee Sriwongchai ; Pattavipha Songkumarn ; Nonglak Parinthawong ; Kulchana Darwell ; Sucheela Talumphai ; Piyama Tasanasuwan ; Chatchawan JantasuriyaratSource :
- Journal of genetics [ 0973-7731 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Ascomycota, Séquence nucléotidique.
- isolement et purification : Ascomycota.
- Facteurs de virulence, Oryza, Phylogenèse, Réaction de polymérisation en chaîne, Variation génétique.
English descriptors
- KwdEn :
- MESH :
- chemical : Virulence Factors.
- genetics : Ascomycota, Base Sequence.
- isolation & purification : Ascomycota.
- Genetic Variation, Oryza, Phylogeny, Polymerase Chain Reaction.
Abstract
Rice blast disease, caused by Magnaporthe oryzae, is one of the most importance diseases of rice production worldwide. The keyrole of defense mechanism to combat this fungus in rice follows the gene-for-gene concept, which a plant resistant (R) gene product recognizes a fungal avirulent (AVR) effector and triggers the hypersensitive response. However, the AVR genes have been shown to be rapidly evolving resulting in high level of genetic diversity. The aims of this study were to examine the nucleotide sequence variation of AVR-Pita1 gene in Thai rice blast isolates and to identify the severity of blast disease using isogenic line of Pita gene. Seventy-six rice blast isolates collected from different parts of Thailand were used. Gene specific primers for AVR-Pita1 gene coding sequence were designed and used for identifying the genetic diversity of AVR-Pita1 gene by PCR amplification and sequencing. The obtained sequences were analysed for genetic variation and genetic relationship. Our results revealed the association between the sequence variations of AVR-Pita1 and selective forces from Pita gene. This phenomenon demonstrated the coevolution between rice blast resistant gene in rice and avirulent gene in blast fungus. The information about variation and evolutionary mechanisms of AVR gene obtained from this study can be used in rice blast resistant breeding programme.
PubMed: 32529988
Affiliations:
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first="Tanee" last="Sriwongchai">Tanee Sriwongchai</name></author><author><name sortKey="Songkumarn, Pattavipha" sort="Songkumarn, Pattavipha" uniqKey="Songkumarn P" first="Pattavipha" last="Songkumarn">Pattavipha Songkumarn</name></author><author><name sortKey="Parinthawong, Nonglak" sort="Parinthawong, Nonglak" uniqKey="Parinthawong N" first="Nonglak" last="Parinthawong">Nonglak Parinthawong</name></author><author><name sortKey="Darwell, Kulchana" sort="Darwell, Kulchana" uniqKey="Darwell K" first="Kulchana" last="Darwell">Kulchana Darwell</name></author><author><name sortKey="Talumphai, Sucheela" sort="Talumphai, Sucheela" uniqKey="Talumphai S" first="Sucheela" last="Talumphai">Sucheela Talumphai</name></author><author><name sortKey="Tasanasuwan, Piyama" sort="Tasanasuwan, Piyama" uniqKey="Tasanasuwan P" first="Piyama" last="Tasanasuwan">Piyama Tasanasuwan</name></author><author><name sortKey="Jantasuriyarat, Chatchawan" sort="Jantasuriyarat, Chatchawan" uniqKey="Jantasuriyarat C" 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Parinthawong</name></author><author><name sortKey="Darwell, Kulchana" sort="Darwell, Kulchana" uniqKey="Darwell K" first="Kulchana" last="Darwell">Kulchana Darwell</name></author><author><name sortKey="Talumphai, Sucheela" sort="Talumphai, Sucheela" uniqKey="Talumphai S" first="Sucheela" last="Talumphai">Sucheela Talumphai</name></author><author><name sortKey="Tasanasuwan, Piyama" sort="Tasanasuwan, Piyama" uniqKey="Tasanasuwan P" first="Piyama" last="Tasanasuwan">Piyama Tasanasuwan</name></author><author><name sortKey="Jantasuriyarat, Chatchawan" sort="Jantasuriyarat, Chatchawan" uniqKey="Jantasuriyarat C" first="Chatchawan" last="Jantasuriyarat">Chatchawan Jantasuriyarat</name></author></analytic><series><title level="j">Journal of genetics</title><idno type="eISSN">0973-7731</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ascomycota (genetics)</term><term>Ascomycota (isolation & purification)</term><term>Base Sequence (genetics)</term><term>Genetic Variation (MeSH)</term><term>Oryza (MeSH)</term><term>Phylogeny (MeSH)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Virulence Factors (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Ascomycota (génétique)</term><term>Ascomycota (isolement et purification)</term><term>Facteurs de virulence (MeSH)</term><term>Oryza (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Séquence nucléotidique (génétique)</term><term>Variation génétique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Virulence Factors</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Ascomycota</term><term>Base Sequence</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Ascomycota</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Ascomycota</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Ascomycota</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genetic Variation</term><term>Oryza</term><term>Phylogeny</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Facteurs de virulence</term><term>Oryza</term><term>Phylogenèse</term><term>Réaction de polymérisation en chaîne</term><term>Variation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Rice blast disease, caused by <i>Magnaporthe oryzae</i>, is one of the most importance diseases of rice production worldwide. The keyrole of defense mechanism to combat this fungus in rice follows the gene-for-gene concept, which a plant resistant (<i>R</i>) gene product recognizes a fungal avirulent (<i>AVR</i>) effector and triggers the hypersensitive response. However, the <i>AVR</i> genes have been shown to be rapidly evolving resulting in high level of genetic diversity. The aims of this study were to examine the nucleotide sequence variation of <i>AVR-Pita1</i> gene in Thai rice blast isolates and to identify the severity of blast disease using isogenic line of Pita gene. Seventy-six rice blast isolates collected from different parts of Thailand were used. Gene specific primers for <i>AVR-Pita1</i> gene coding sequence were designed and used for identifying the genetic diversity of <i>AVR-Pita1</i> gene by PCR amplification and sequencing. The obtained sequences were analysed for genetic variation and genetic relationship. Our results revealed the association between the sequence variations of <i>AVR-Pita1</i> and selective forces from <i>Pita</i> gene. This phenomenon demonstrated the coevolution between rice blast resistant gene in rice and avirulent gene in blast fungus. The information about variation and evolutionary mechanisms of <i>AVR</i> gene obtained from this study can be used in rice blast resistant breeding programme.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32529988</PMID><DateCompleted><Year>2020</Year><Month>10</Month><Day>08</Day></DateCompleted><DateRevised><Year>2020</Year><Month>10</Month><Day>08</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">0973-7731</ISSN><JournalIssue CitedMedium="Internet"><Volume>99</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Journal of genetics</Title><ISOAbbreviation>J Genet</ISOAbbreviation></Journal><ArticleTitle>High nucleotide sequence variation of avirulent gene, <i>AVR-Pita1</i>, in Thai rice blast fungus population.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">45</ELocationID><Abstract><AbstractText>Rice blast disease, caused by <i>Magnaporthe oryzae</i>, is one of the most importance diseases of rice production worldwide. The keyrole of defense mechanism to combat this fungus in rice follows the gene-for-gene concept, which a plant resistant (<i>R</i>) gene product recognizes a fungal avirulent (<i>AVR</i>) effector and triggers the hypersensitive response. However, the <i>AVR</i> genes have been shown to be rapidly evolving resulting in high level of genetic diversity. The aims of this study were to examine the nucleotide sequence variation of <i>AVR-Pita1</i> gene in Thai rice blast isolates and to identify the severity of blast disease using isogenic line of Pita gene. Seventy-six rice blast isolates collected from different parts of Thailand were used. Gene specific primers for <i>AVR-Pita1</i> gene coding sequence were designed and used for identifying the genetic diversity of <i>AVR-Pita1</i> gene by PCR amplification and sequencing. The obtained sequences were analysed for genetic variation and genetic relationship. Our results revealed the association between the sequence variations of <i>AVR-Pita1</i> and selective forces from <i>Pita</i> gene. This phenomenon demonstrated the coevolution between rice blast resistant gene in rice and avirulent gene in blast fungus. The information about variation and evolutionary mechanisms of <i>AVR</i> gene obtained from this study can be used in rice blast resistant breeding programme.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Damchuay</LastName><ForeName>Katanyutita</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Faculty of Science, Department of Genetics, Kasetsart University, Chatuchak, Bangkok 10900, Thailand. fscicwj@ku.ac.th.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Longya</LastName><ForeName>Apinya</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Sriwongchai</LastName><ForeName>Tanee</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Songkumarn</LastName><ForeName>Pattavipha</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Parinthawong</LastName><ForeName>Nonglak</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Darwell</LastName><ForeName>Kulchana</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Talumphai</LastName><ForeName>Sucheela</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Tasanasuwan</LastName><ForeName>Piyama</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Jantasuriyarat</LastName><ForeName>Chatchawan</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>India</Country><MedlineTA>J Genet</MedlineTA><NlmUniqueID>2985113R</NlmUniqueID><ISSNLinking>0022-1333</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D037521">Virulence Factors</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="N">Ascomycota</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="Y">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012275" MajorTopicYN="N">Oryza</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D037521" MajorTopicYN="N">Virulence Factors</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>6</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>6</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32529988</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Thaïlande</li></country></list><tree><noCountry><name sortKey="Darwell, Kulchana" sort="Darwell, Kulchana" uniqKey="Darwell K" first="Kulchana" last="Darwell">Kulchana Darwell</name><name sortKey="Jantasuriyarat, Chatchawan" sort="Jantasuriyarat, Chatchawan" uniqKey="Jantasuriyarat C" first="Chatchawan" last="Jantasuriyarat">Chatchawan Jantasuriyarat</name><name sortKey="Longya, Apinya" sort="Longya, Apinya" uniqKey="Longya A" first="Apinya" last="Longya">Apinya Longya</name><name sortKey="Parinthawong, Nonglak" sort="Parinthawong, Nonglak" uniqKey="Parinthawong N" first="Nonglak" last="Parinthawong">Nonglak Parinthawong</name><name sortKey="Songkumarn, Pattavipha" sort="Songkumarn, Pattavipha" uniqKey="Songkumarn P" first="Pattavipha" last="Songkumarn">Pattavipha Songkumarn</name><name sortKey="Sriwongchai, Tanee" sort="Sriwongchai, Tanee" uniqKey="Sriwongchai T" first="Tanee" last="Sriwongchai">Tanee Sriwongchai</name><name sortKey="Talumphai, Sucheela" sort="Talumphai, Sucheela" uniqKey="Talumphai S" first="Sucheela" last="Talumphai">Sucheela Talumphai</name><name sortKey="Tasanasuwan, Piyama" sort="Tasanasuwan, Piyama" uniqKey="Tasanasuwan P" first="Piyama" last="Tasanasuwan">Piyama Tasanasuwan</name></noCountry><country name="Thaïlande"><noRegion><name sortKey="Damchuay, Katanyutita" sort="Damchuay, Katanyutita" uniqKey="Damchuay K" first="Katanyutita" last="Damchuay">Katanyutita Damchuay</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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